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      Human Complement component C6(C6) ELISA kit

      Datasheet
      • 中文名稱:
        Human Complement component C6(C6) ELISA kit
      • 貨號:
        CSB-EL004036HU
      • 規格:
        96T
      • 價格:
        3600
      • 其他:

      產品詳情

      • 別名:
        AW111623 ELISA Kit; C6 ELISA Kit; CO6_HUMAN ELISA Kit; Complement component 6 ELISA Kit; Complement component C6 ELISA Kit
      • 縮寫:
        C6
      • Uniprot No.:
      • 種屬:
        Homo sapiens (Human)
      • 樣本類型:
        serum, plasma, tissue homogenates
      • 檢測范圍:
        62.5 pg/mL-4000 pg/mL
      • 靈敏度:
        15.6 pg/mL
      • 反應時間:
        1-5h
      • 樣本體積:
        50-100ul
      • 檢測波長:
        450 nm
      • 研究領域:
        Immunology
      • 測定原理:
        quantitative
      • 測定方法:
        Sandwich
      • 精密度:
        Intra-assay Precision (Precision within an assay): CV%<8%        
        Three samples of known concentration were tested twenty times on one plate to assess.    
        Inter-assay Precision (Precision between assays): CV%<10%        
        Three samples of known concentration were tested in twenty assays to assess.      
                       
      • 線性度:
        To assess the linearity of the assay, samples were spiked with high concentrations of human C6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
          Sample Serum(n=4)    
        1:10000 Average % 88    
        Range % 81-92    
        1:20000 Average % 95    
        Range % 91-105    
        1:40000 Average % 97    
        Range % 92-101    
        1:80000 Average % 93    
        Range % 86-98    
      • 回收率:
        The recovery of human C6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
         
        Sample Type Average % Recovery Range    
        Serum (n=5) 92 85-98    
        EDTA plasma (n=4) 95 90-100    
                       
                       
      • 標準曲線:
        These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
         
        pg/ml OD1 OD2 Average Corrected    
        4000 2.021 2.107 2.064 1.940    
        2000 1.745 1.784 1.765 1.641    
        1000 1.318 1.329 1.324 1.200    
        500 0.902 0.894 0.898 0.774    
        250 0.651 0.668 0.660 0.536    
        125 0.489 0.492 0.491 0.367    
        62.5 0.337 0.341 0.339 0.215    
        0 0.122 0.125 0.124      
      • 貨期:
        5-7 working days

      靶點詳情

      • 功能:
        Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.
      • 基因功能參考文獻:
        1. Our study provided novel evidence that genetic variations in complement genes C6 and MASP1were associated with preeclampsia risk, and that the risk varied by preeclampsia subtypes PMID: 27405496
        2. recipient C6 rs9200 polymorphism is associated with HCC recurrence after OLT, and improves the predictive value of clinical models. PMID: 27173880
        3. Structure of complement C6 suggests a mechanism for initiation and unidirectional, sequential assembly of membrane attack complex (MAC). PMID: 22267737
        4. finding of very high serious morbidity in patients with complete C6 deficiency due to meningococcal infections PMID: 22288589
        5. Genetic polymorphisms in C6 gene do not influence the risk of aspirin hypersensitivity in Korean asthmatic patients. PMID: 21704099
        6. produced by trophoblast cells PMID: 19665237
        7. The most common defect, which leads to an increased susceptibility to Neisseria meningitidis infections in the Western Cape, is 879delG. PMID: 17257682

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      • 相關疾?。?/div>
        Complement component 6 deficiency (C6D)
      • 亞細胞定位:
        Secreted
      • 蛋白家族:
        Complement C6/C7/C8/C9 family
      • 數據庫鏈接:

        HGNC: 1339

        OMIM: 217050

        KEGG: hsa:729

        STRING: 9606.ENSP00000263413

        UniGene: Hs.481992



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