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      Recombinant Human Novel Coronavirus Spike glycoprotein(S), partial

      In Stock
      • 貨號:
        CSB-YP3324GMY1
      • 規格:
      • 圖片:
        • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
          Predicted band size: 38.2 kDa
          Observed band size:
          (a) 68 kDa before EndoH Digestion
          (b) 38 kDa after EndoH Digestion

        • Activity
          Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.

        • Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml.

        • Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A0GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 13.48-19.50 ng/ml.

        • SARS-CoV-2 Spike protein RBD his/sumostar tag (CSB-YP3324GMY1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 100 nM as detected by LSPR Assay.

        • SARS-CoV-2 Spike protein RBD His/Sumostar Tag (CSB-YP3324GMY1) captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with an affinity constant of 28.2nM as detected by LSPR Assay.

      • 其他:

      產品詳情

      • 純度:
        >Greater than 90% as determined by SDS-PAGE.
      • 生物活性:
        >①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml.③Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A0GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 13.48-19.50 ng/ml.④SARS-CoV-2 Spike protein RBD his/sumostar tag (CSB-YP3324GMY1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 100 nM as detected by LSPR Assay.⑤SARS-CoV-2 Spike protein RBD His/Sumostar Tag (CSB-YP3324GMY1) captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with an affinity constant of 28.2nM as detected by LSPR Assay.
      • Uniprot No.:
      • 種屬:
        Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
      • 蛋白長度:
        Partial
      • 來源:
        Yeast
      • 分子量:
        38.2 kDa
      • 表達區域:
        319-541aa
      • 氨基酸序列
        RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
      • 蛋白標簽:
        N-terminal 6xHis-sumostar-tagged
      • 產品提供形式:
        Lyophilized powder
        Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
      • 緩沖液:
        If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
        Note: If you have any special requirement for the glycerol content, please remark when you place the order.
        If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
      • 儲存條件:
        Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
      • 保質期:
        The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
        Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
      • 貨期:
        Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
      • 注意事項:
        Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
      • Datasheet & COA:
        Please contact us to get it.


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