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      Proteinase?K?Recombinant?Protein

      In Stock
      • 貨號:
        CSB-AP361972TIQ
      • 規格:
      • 圖片:
        • SDS-PAGE

          (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
          According to the left SDS-PAGE image, the purity of Proteinase K is 95%+ and the molecular weight is 28.9 kDa.

        • Detect Nucleic acid residue by agarose gel electrophores

        • Detect DNase residue by agarose gel electrophores

        • Detect RNase residue by agarose gel electrophores

        • Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated >30U/mg. Unit definition:One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C

        • The Enzyme Activity Stability of Proteinase K

          The Proteinase K powder was stored at 4℃. And its enzyme activity was assayed every month over the past year.
          The left chart shows how its enzyme activity changes in a year. It turns out the enzyme activity is maintained more than 95%, which indicates that proteinase K is very stable.

        • The Proteinase K activity comparison of 3 vendors and CUSABIO

          CUSABIO performed a Proteinase K activity comparison test. As the left chart shows, the activity of CUSABIO Proteinase K is better than that of the 3 vendors under the same testing conditions.

      • 其他:

      產品詳情

      • 純度:
        Greater than 95% as determined by SDS-PAGE.
      • 生物活性:
        Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated >30U/mg.
      • 基因名:
        PROK
      • Uniprot No.:
      • 別名:
        Endopeptidase K,Tritirachium alkaline proteinase
      • 種屬:
        Tritirachium album (Engyodontium album)
      • 分子量:
        28.9 kDa
      • 產品提供形式:
        Lyophilized powder
        Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
      • 緩沖液:
        If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
        Note: If you have any special requirement for the glycerol content, please remark when you place the order.
        If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
      • 復溶:
        We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
      • 儲存條件:
        Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
      • 保質期:
        The shelf life of the lyophilized form is 24 months at -20°C/-80°C, 18 months at 4°C, and 12 months at room temperature.
      • 貨期:
        Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
      • 注意事項:
        Repeated freezing and thawing is not recommended.
      • 產品描述:

        Proteinase K, originally isolated from the mold Tritirachium album, is a serine protease with broad substrate specificity and relatively high proteolytic activity. It preferentially cleaves ester and peptide bonds adjacent to the C-termini of hydrophobic, aliphatic, or aromatic amino acids. CUSABIO's recombinant proteinase K is highly pure determined by SDS-PAGE and reached up to 95%. It is characterized by high purity, sterility, no bio-burden, and no presence of DNAse, RNAse, DNA, and RNA contaminants. Its enzyme activity was calculated >30U/mg using the absorbance A275 as the vertical axis and different concentrations of a tyrosine as the horizontal axis. The working concentration of proteinase K, usually determined by the temperature, PH, and whether the buffer used contains SDS and urea, is 20mg/mL. This recombinant proteinase K protein is in-stock now.

        Proteinase K has a wide range of applications. In nucleic acid extraction and isolation, proteinase K can be used to degrade Dnase/Rnase and DNA-bound histones and lyse cells or tissues. It also digests protein impurities especially endotoxin and separates target nucleic acids from proteins in biological samples, thus improving the purity and safety of nucleic acids and production efficiency. The nucleic acids isolated by proteinase K is often used for the amplification reaction. Denaturing agents such as SDS (1%) can increase the enzymatic activity of proteinase K. In in-situ hybridization, it removes the proteins around the target DNA to facilitate probe infiltration and improves detection sensitivity. Proteinase K is also used to analyze the membrane structure through the modification of proteins on the cell surface. The nucleic acid-based real-time reverse transcriptase-polymerase chain reaction (RT-PCR) test is the primary tool to diagnose the SRAS-CoV-2 infection. Proteinase K is often used to remove unwanted proteins present in the nasal/pharyngeal swab samples and inactivate nucleases that could degrade DNA or RNA during isolation and purification procedures, thus increasing the yield of extracted RNA and improving the detection of viral RNA by RT-PCR.

      • Datasheet & COA:
        Please contact us to get it.

      問答及客戶評論

       常見問題解答
      Q:

      蛋白酶K粉末怎樣溶解?

      A:
      蛋白酶K粉末可溶于20mM Tris-HCl、200mM NaCl、50%甘油,pH8.4的混合溶液中。溶解后,可在4℃下保存一年。如果蛋白酶K能在15天內用完,可以直接加入純水和50%甘油使用。
      Q:

      我們蛋白酶K的長期儲存溫度是多少?

      A:
      T我們的蛋白酶K呈粉末狀,可在4℃保存18個月,在-20℃保存2年。
      Q:

      對于液體蛋白酶K和粉末蛋白酶K,反復凍融會影響它們的活性嗎?對哪個的影響更大?

      A:
      無論蛋白酶K是粉末還是液體,我們都不建議反復凍融,而且所有的蛋白產品都不適合反復凍融。與液體蛋白酶K相比,反復凍融對粉末的蛋白酶K影響會小一些,但是要避免吸潮。
      Q:

      蛋白酶K的推薦工作濃度是多少?

      A:
      我們的蛋白酶K產品推薦的工作濃度是20mg/mL。
      Q:

      蛋白酶K活性的最適溫度是多少?怎樣滅活蛋白酶K?

      A:
      蛋白酶K活性的最適溫度在50~65?C。溫度超過65℃后,隨著溫度的升高,蛋白酶K的活性會降低。若將蛋白酶K置于95℃加熱到10分鐘,蛋白酶K就會失活。但值得注意的是,加熱并不能完全使蛋白酶K失活。
      Q:

      EDTA可以使蛋白酶K失活嗎?

      A:
      在DNA或RNA純化過程中,EDTA之所以與蛋白酶K一起使用是為了去除鈣離子。但是因為鈣離子與蛋白酶K的穩定性有關,而添加EDTA會影響鈣離子,因此添加EDTA在一定程度上可以降低蛋白酶K的活性。

      靶點詳情

      • 功能:
        Hydrolyzes keratin at aromatic and hydrophobic residues.
      • 蛋白家族:
        Peptidase S8 family


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