COVID-19 Antibody Reagents

SARS-CoV-2抗體

新冠病毒引起的新冠肺炎疫情此起彼伏,對全球的醫療衛生系統和經濟帶來了巨大影響,同時也給新冠疫苗、治療藥物以及診斷帶來了新的挑戰。武漢華美生物一直密切關注新冠病毒的進化情況,目前已開發了大量高質量SARS-CoV-2相關抗體試劑,包括N抗體、S抗體、S RBD納米抗體、N抗體對和S RBD抗體對等。

其中,N抗體與S抗體可用于新冠病毒致病性機理研究,可通過WB定性檢測新冠抗原;N抗體對和S RBD抗體對可用于新冠試劑盒的開發;另外,部分抗體驗證了中和試驗和主流突變株檢測,可作為質控品,應用于針對突變株的中和抗體篩選、重組蛋白疫苗研發、疫苗接種后血清中的中和抗體檢測等。

華美特色

  • 抗體試劑類型多
  • 高特異性
  • 多種應用場景驗證
  • 多個主流突變株驗證

部分應用場景及驗證數據

● WB定性抗原檢測

Positive WB detected in: His tag-tagged SARS-CoV-2 N recombinant protein (CSB-EP3325GMY); SARS-CoV-2 N antibody at 1:1000 (CSB-RA33255A1GMY)

Positive WB detected in: SARS-CoV-2 N recombinant protein (CSB-EP3325GMY) at 40ng, 20ng, 10ng; All lanes N antibody (CSB-MA33255A0m) at 1:1000

● 功能性ELISA驗證抗原-抗體結合活性

Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/mL can bind SARS-CoV-2-N (CSB-RA33255A0GMY) Antibody, the EC50 is 6.892 ng/mL.

Immobilized SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-MA33245A0m), the EC50 is 32.59 to 39.33 ng/mL.

● 用于膠體金(GICA)快速檢測

In the GICA detection system, the background of antibody (CSB-RA33255A2GMY) is clean, the detection limit can be as low as 15.625ng/mL (1.09ng/0.07mL), and the sensitivity is very good.

In the GICA detection system, the background of antibody (CSB-RA33245A0GMY) is clean, the detection limit can be as low as 13.94ng/mL (0.976ng/0.07mL), and the sensitivity is very good.

● 抗體對經S-ELISA驗證

SARS-CoV-2 N Antibody Pair 1 (CSB-EAP33255A1) is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA).

SARS-CoV-2 Spike RBD Antibody Pair 1 (CSB-EAP33245) is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA).

● 經中和試驗驗證中和抗體

Binding signal of SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) and ACE2 protein-HRP conjugate (CSB-MP866317HU) was inhibited by S Antibody (CSB-RA33245A1GMY) with the IC50 is 2.38 μg/mL

Binding signal of SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) and ACE2-HRP (CSB-YP3324GMY1) was inhibited by SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with the IC50 is 0.1074 μg/mL.

● 多個突變株識別驗證

The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/mL can bind N Recombinant Antibody (CSB-RA33255A1GMY), the EC50 of N Recombinant Antibody.

The Binding Activity of S protein with S Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized S at 2 μg/mL can bind S Recombinant Antibody, the EC50 of S Recombinant Antibody (CSB-RA33245A1GMY).

產品列表

● N antibody

貨號 產品名稱 反應種屬 應用類型
CSB-RA33255A1GMY N Antibody SARS-CoV-2 ELISA, WB, GICA
CSB-RA33255A2GMY N Antibody SARS-CoV-2 ELISA
CSB-MA33255A0m N Antibody GICA Mouse
CSB-PA33254A0Rb N Antibody GICA Rabbit
CSB-RA33255A0GMY N Antibody SARS-CoV-2 ELISA, GICA
CSB-RA33255B1GMY N Antibody, HRP conjugated SARS-CoV-2 ELISA
CSB-RA33255C1GMY N Antibody, FITC conjugated SARS-CoV-2 ELISA
CSB-RA33255D1GMY N Antibody, Biotin conjugated SARS-CoV-2 ELISA
CSB-MA33255A1m N Antibody GICA Mouse
CSB-MA33255A2m N Antibody GICA Mouse
CSB-PA33254B0Rb N Antibody, HRP conjugated SARS-CoV-2 ELISA
CSB-PA33254C0Rb N Antibody, FITC conjugated SARS-CoV-2 ELISA
CSB-PA33254D0Rb N Antibody, Biotin conjugated SARS-CoV-2 ELISA

● S antibody

貨號 產品名稱 反應種屬 應用類型
CSB-RA33245A1GMY S Antibody SARS-CoV-2 ELISA, GICA, Neutralising
CSB-MA33245A2m S Monoclonal Antibody GICA Mouse
CSB-PA33245YA01GMY S Antibody GICA Rabbit
CSB-RA33245A0GMY S Antibody SARS-CoV-2 ELISA, GICA
CSB-RA33245B1GMY S Antibody, HRP conjugated SARS-CoV-2 ELISA
CSB-RA33245C1GMY S Antibody, FITC conjugated SARS-CoV-2 ELISA
CSB-RA33245D1GMY S Antibody, Biotin conjugated SARS-CoV-2 ELISA
CSB-MA33245A0m S Monoclonal Antibody GICA Mouse
CSB-MA33245A1m S Monoclonal Antibody GICA Mouse
CSB-PA33245YB01GMY S Antibody SARS-CoV-2 ELISA
CSB-PA33245YC01GMY S Antibody SARS-CoV-2 ELISA
CSB-PA33245YD01GMY S Antibody SARS-CoV-2 ELISA
CSB-PA33245YA11GMY S Antibody GICA Rabbit
CSB-PA33245YB11GMY S Antibody SARS-CoV-2 ELISA
CSB-PA33245YC11GMY S Antibody SARS-CoV-2 ELISA
CSB-PA33245YD11GMY S Antibody SARS-CoV-2 ELISA

● N Antibody Pair

貨號 產品名稱 反應種屬 應用類型
CSB-EAP33255A2 SARS-CoV-2 N Antibody Pair 2 SARS-CoV-2 S-ELISA
CSB-EAP33255A1 SARS-CoV-2 N Antibody Pair 1 SARS-CoV-2 S-ELISA
CSB-EAP33255A3 SARS-CoV-2 N Antibody Pair 3 SARS-CoV-2 S-ELISA
CSB-EAP33255A4 SARS-CoV-2 N Antibody Pair 4 SARS-CoV-2 S-ELISA

● S RBD Nanobody Antibody

貨號 產品名稱 反應種屬 應用類型
CSB-RA33245A2GMY SARS-CoV-2 RBD Nanobody SARS-CoV-2 ELISA, GICA, Neutralising
CSB-RA33245B2GMY SARS-CoV-2 RBD Nanobody, HRP conjugated SARS-CoV-2 ELISA
CSB-RA33245C2GMY SARS-CoV-2 RBD Nanobody, FITC conjugated SARS-CoV-2 ELISA
CSB-RA33245D2GMY SARS-CoV-2 RBD Nanobody, Biotin conjugated SARS-CoV-2 ELISA

● S RBD Antibody Pair

貨號 產品名稱 反應種屬 應用類型
CSB-EAP33245 SARS-CoV-2 Spike RBD Antibody Pair 1 SARS-CoV-2 S-ELISA


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